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Differentiation of Bovine Spermatogonial Stem
Cells into Osteoblasts
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By
Babak Qasemi-Panahi , Parviz Tajik , Mansoureh Movahedin , Gholamali Moghaddam Younes Barzgar , and Hamed Heidari-Vala © 2011. Avicenna Research Institute
Avicenna Journal of Medical Biotechnology (AJMB), Volume 3, Issue 3
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149-153 Page(s) |
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Abstract
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Spermatogonial Stem Cell (SSC) technologies provide multiple opportunities
for research in the field of biotechnology and regenerative medicine. The
therapeutic use of Embryonic Stem Cells (ESCs) is restricted due to severe eth-
ical and immunological concerns. Therefore, we need a new pluripotent cell
type. Despite well-known role of germ cells in the gametogenesis, some facts
apparently show their multipotentiality. In the present study, bovine SSCs
were co-cultured with Sertoli cell for 7 days. Sertoli cells and SSCs were iden-
tified by Vimentin and Oct-4 immunocytochemical staining method, respect-
ively... |
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Keywords
Differentiation, Osteoblast, Spermatogonial stem cell
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Biogenic Silver Nanoparticles by Gelidiella
acerosa Extract and their
Antifungal Effects
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By
Marimuthu Vivek, Palanisamy Senthil Kumar, Sesurajan Steffi, and Sellappa Sudha © 2011. Avicenna Research Institute
Avicenna Journal of Medical Biotechnology (AJMB), Volume 3, Issue 3
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143.-148 Page(s) |
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Abstract
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The synthesis, characterization and application of biologically synthesized
nanomaterials are an important aspect in nanotechnology. The present study
deals with the synthesis of silver nanoparticles (Ag-NPs) using the aqueous
extract of red seaweed Gelidiella acerosa as the reducing agent to study the
antifungal activity. The formation of Ag-NPs was confirmed by UV-Visible
Spectroscopy, X-Ray Diffraction (XRD) pattern, Scanning Electron Microscopy
(SEM) and Transmission Electron Microscopy (TEM). The synthesized Ag-NPs
was predominately spherical in shape and polydispersed... |
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Keywords
Gelidiella acerosa, Silver nanoparticles, Nanotechnology, Seaweed
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Construction and In vitro Expression Analyses of a
DNA Plasmid Encoding
Dense Granule GRA5 Antigen of Toxoplasma gondii
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By
Jalal Babaie, Ghazaleh Sadeghiani, and Majid Golkar © 2011. Avicenna Research Institute
Avicenna Journal of Medical Biotechnology (AJMB), Volume 3, Issue 3
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135-141 Page(s) |
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Abstract
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Toxoplasmosis is an infection caused by the protozoan parasite Toxoplasma
gondii (T.gondii) throughout the world. Although usually asymptomatic, the
infection can cause serious medical problems in immunocompromised individ-
uals and fetuses. Toxoplasmosis also causes considerable economic loss because
of abortion in livestock. DNA vaccination is a promising approach against
intracellular parasites such as T.gondii. The goal of this study was to construct
and evaluate functionality of a mammalian plasmid expressing GRA5 anti-
gen of T... |
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Keywords
Toxoplasma gondii, DNA vaccine, GRA5 protein
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Expression and Purification of Functionally Active
Recombinant Human
Alpha 1-Antitrypsin in Methylotrophic Yeast Pichia
pastoris
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By
Sareh Arjmand
, Elham Bidram
, Abbas Sahebghadam Lotfi
, Mehdi Shamsara
and Seyed Javad Mowla
© 2011. Avicenna Research Institute
Avicenna Journal of Medical Biotechnology (AJMB), Volume 3, Issue 3
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127-134 Page(s) |
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Abstract
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Human alpha 1-antitrypsin (AAT) cDNA was obtained from HepG2 cell lines.
After PCR and construction of expression vector pPICZ |
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Keywords
Alpha 1-antitrypsin, Pichia pastoris, Protease inhibitors,
Recombinant proteins
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Comparative Expression Profile of Orphan Receptor
Tyrosine Kinase ROR1 in
Iranian Patients with Lymphoid and Myeloid
Leukemias
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By
Mahdi Shabani , Hossein Asgarian Omran , Mohammad Hojjat Farsangi , Parvaneh Vossough ,
Ramazan A. Sharifian, Gholam R. Toughe , Seyed Mohsen Razavi , Jalal Khoshnoodi ,
Mahmood Jeddi-Tehrani , Hodjatallah Rabbani , and Fazel Shokri © 2011. Avicenna Research Institute
Avicenna Journal of Medical Biotechnology (AJMB), Volume 3, Issue 3
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119-125 Page(s) |
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Abstract
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It has recently been shown that ROR1, a member of the receptor tyrosine
kinase family, is overexpressed in leukemic B cells of Chronic Lymphocytic
Leukemia (CLL) and a subset of Acute Lymphoblastic Leukemia (ALL). In this
comparative study the expression profile of ROR1 mRNA was investigated in
Iranian patients with CLL and Acute Myelogenous Leukemia (AML) and the
results were compared with those previously reported in our Iranian ALL
patients. RT-PCR was performed on bone marrow and/or peripheral blood
samples of 84 CLL and 12 AML patients... |
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Keywords
Acute myelogenous leukemia, Chronic lymphocytic leukemia,
ROR1, RT-PCR
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Genetically Modified Foods and Social Concerns
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By
Behrokh Mohajer Maghari
and Ali M. Ardekani © 2011. Avicenna Research Institute
Avicenna Journal of Medical Biotechnology (AJMB), Volume 3, Issue 3
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109-117 Page(s) |
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Abstract
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Biotechnology is providing us with a wide range of options for how we can use
agricultural and commercial forestry lands. The cultivation of genetically
modified (GM) crops on millions of hectares of lands and their injection into
our food chain is a huge global genetic experiment involving all living beings.
Considering the fast pace of new advances in production of genetically mod-ified crops, consumers, farmers and policymakers worldwide are challenged to
reach a consensus on a clear vision for the future of world food supply... |
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Keywords
Food, Genetically Engineered, Genetically modified, GMOs, Health,
Humans
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Nephroprotective and Nitric oxide Scavenging
Activity of Tubers of
Momordica tuberosa in Rats
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By
Pramod Kumar , Devala Rao G. , Lakshmayya , and Ramachandra Setty S. © 2011. Avicenna Research Institute
Avicenna Journal of Medical Biotechnology (AJMB), Volume 3, Issue 2
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87-93 Page(s) |
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Abstract
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e, tissue glutathione and lipid peroxidation in administered doses.
The extract exhibited free radical scavenging activity in dose dependant
manner. And 100 |
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Keywords
Blood urea nitrogen, Cisplatin, Cucurbitaceae, Kidney failure, Lipid
peroxidation, Nitric oxide
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Peptide-based Polyclonal Antibody Production
against P110 Protein of
Mycoplasma genitalium
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By
Omid Zarei , Gholam Reza Irajian , Amir Hassan Zarnani , Leili Chamani-Tabriz
Shaghayegh Emami , Mahmood Jeddi-Tehrani , and Hodjattallah Rabbani © 2011. Avicenna Research Institute
Avicenna Journal of Medical Biotechnology (AJMB), Volume 3, Issue 2
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79-85 Page(s) |
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Abstract
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Mycoplasma genitalium (M.genitalium) is a sexually transmitted pathogen.
Detection of this microorganism in clinical specimens by culture is rather
difficult and time consuming. The aim of this study was to produce polyclonal
antibody against a synthetic peptide from P110 protein of M.genitalium in
order to develop a diagnostic tool for detection of this microorganism in
clinical specimens. A synthetic peptide from P110 protein was conjugated to
Keyhole Limpet Hemocyanin (KLH) and used for immunization of a white
New Zealand rabbit... |
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Keywords
Antibodies, Mycoplasma genitalium, Peptides
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Expression and Single-step Purification of GRA8
Antigen of Toxoplasma
gondii in Escherichia coli
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By
Jalal Babaie
, Mandana Miri
, Ghazaleh Sadeghiani
, Mehrak Zare ,
Ghader Khalili
, and Majid Golkar © 2011. Avicenna Research Institute
Avicenna Journal of Medical Biotechnology (AJMB), Volume 3, Issue 2
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67-77 Page(s) |
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Abstract
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Diagnosis of Toxoplasma gondii (T.gondii) infection is of great medical import-
ance especially for pregnant women and immunosuppressed patients. Nu-
merous studies have shown that the recombinant production of several toxo-
plasma antigens, including dense granule antigens (GRAs) has a great poten-
tial as diagnostic reagents. Previous studies reported expression of amino
terminal GRA8 protein in fusion with large tags. In the present study, we
produced truncated GRA8 (GRA8), excluded from the signal peptide and C-
terminal transmembrane domain, with a short fusion tag in Escherichia coli
(E... |
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Keywords
Enzyme-linked immunosorbent assay, Gene expression, GRA8 protein,
Immunoglobulin M, Toxoplasma gondii
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Antidiarrheal, Antioxidant and Antimicrobial
Activities of the
Musa sapientum Seed
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By
M. Sarowar Hossain
, M. Badrul Alam
, M. Asadujjaman
, Ronok Zahan
M. Monirul Islam
, M. Ehsanul H. Mazumder
, and Md. Ekramul Haque © 2011. Avicenna Research Institute
Avicenna Journal of Medical Biotechnology (AJMB), Volume 3, Issue 2
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95-105 Page(s) |
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Abstract
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Musa sapientum (M.sapientum) commonly known as banana is widely used
in Bangladeshi folk medicine for the treatment of various ailments including
diarrhea. Hence, the present study was designed to investigate antidiarrheal,
antioxidant and antibacterial potential of the methanolic extract of M.sapien-
tum seed (MMSS). The extract was studied for antidiarrheal property using
castor oil and magnesium sulfate induced diarrheal model and charcoal
induced gastrointestinal motility test in mice. Total phenolic and flavonoids
content, total antioxidant activity, scavenging of 1,1-diphenyl-2-picrylhydrazyl
(DPPH) radical, as well as nitric oxide (NO) and assessment of reducing
power were used to evaluate antioxidant potential of MMSS... |
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Keywords
Antibacterial agents, Diarrhea, Free radicals, Musa sapientum
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miR-155 Down Regulation by LNA Inhibitor can
Reduce Cell Growth and
Proliferation in PC12 Cell Line
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By
Fatemeh Kouhkan
, Shaban Alizadeh
, Saeid Kaviani
, Masoud Soleimani
, Ali Akbar Pourfathollah
Naser Amirizadeh
, Saeid Abroun
, Mehrdad Noruzinia
, and Shahin Mohamadi © 2011. Avicenna Research Institute
Avicenna Journal of Medical Biotechnology (AJMB), Volume 3, Issue 2
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61-66 Page(s) |
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Abstract
|
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MicroRNAs (miRNAs) are a class of small non coding regulatory RNAs that
have key functions in multiple cell processes. Deregulation of these tiny
miRNAs is involved in various human diseases. MiR-155 is one of the multi-
functional miRNA that its over-expression has been found to be associated
with different kinds of cancer such as leukemia, breast and colon cancers. It is
thought that deregulation and over-expression of this microRNA may be
associated with PC12 cell proliferation. So, the aim of this study was to
investigate the role of miR-155 expression on PC12 cell growth... |
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Keywords
Apoptosis, miR-155, MTT test, PC12 cells
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How Does Iranians Legal System Protect Human
Vulnerability and Personal
Integrity in Medical Research?
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By
Mohammad Taghi Karoubi
, and Mohammad Mehdi Akhondi
© 2011. Avicenna Research Institute
Avicenna Journal of Medical Biotechnology (AJMB), Volume 3, Issue 2
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51-59 Page(s) |
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Abstract
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The astonishing advance of medical science in recent decades has had endless
advantages for humans, including improved level of health, prevention of
disease and advances in treatment. These advances depend to a great extent
on conducting continuous research. However, besides its enormous advan-tages, the sole interest of medical science undermines the principles of respect
for human vulnerability and personal integrity, in both positive and negative
approaches. The positive approach refers to the people who participate in
research and practice, while the negative approach refers to people who are
deprived of research and practice... |
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Keywords
Bioethics, Integrity, Medical Research, Vulnerability
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Lack of Association between Interleukin 12
C(-1188)A Polymorphism and
Irritable Bowel Syndrome
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By
Elham Barkhordari
, Ali Akbar Amirzargar
, Naser Ebrahimi-Daryani
, Mahdi Mahmou
Bita Ansaripour
, Maryam Alighardashi
, Hamid Reza Ahmadi-Ashtiani
, Mohamma
Bashashati
, and Nima Rezaei © 2011. Avicenna Research Institute
Avicenna Journal of Medical Biotechnology (AJMB), Volume 3, Issue 1
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45-48 Page(s) |
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Abstract
|
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Irritable Bowel Syndrome (IBS) is a functional gastrointestinal disorder,
characterized by recurrent abdominal pain and altered bowel habits. This
study was performed to investigate the important role of interleukin-12 (IL-12)
in intestinal inflammation. For this study seventy one patients with IBS and
140 controls were investigated. The allele and genotype frequencies of IL-12
C(-1188)A were determined using polymerase chain reaction with sequence-
specific primers. The allele A was more common that the allele C in both
groups of patients and controls... |
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Keywords
Genetic polymorphism, Interleukin-12, Irritable bowel syndrome
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Antidiabetic Activity of Aqueous Leaves Extract of
Sesbania sesban (L) Merr.
in Streptozotocin Induced Diabetic Rats
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By
Ramdas B. Pandhare
, B. Sangameswaran
, Popat B. Mohite
, Shantaram G. Khanage © 2011. Avicenna Research Institute
Avicenna Journal of Medical Biotechnology (AJMB), Volume 3, Issue 1
|
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37-43 Page(s) |
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Abstract
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The aqueous leaves extract of Sesbania sesban (L) Merr. (Family: Fabaceae)
was evaluated for its antidiabetic potential on normal and streptozotocin
(STZ)-induced diabetic rats. In the chronic model, the aqueous extract was
administered to normal and STZ- induced diabetic rats at the doses of 250
and 500 mg/kg body weight (b.w.) p.o. per day for 30 days. The fasting
Blood Glucose Levels (BGL), serum insulin level and biochemical data such as
glycosylated hemoglobin, Total Cholesterol (TC), Triglycerides (TG), High
Density Lipoproteins (HDL) and Low Density Lipoproteins (LDL) were evalu-
ated and all were compared to that of the known anti-diabetic drug gliben-
clamide (0... |
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Keywords
Diabetes mellitus, Hyperglycemia, Sesbania sesban, Streptozotocin
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Cloning and Expression of S1 Subunit of Pertussis
Toxin in Escherichia coli
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By
Abolfazl Khafri
, Khosrow Aghaiypour
, Shahin Najar Peerayeh
, and Reihaneh Ghorbani © 2011. Avicenna Research Institute
Avicenna Journal of Medical Biotechnology (AJMB), Volume 3, Issue 1
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19-24 Page(s) |
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Abstract
|
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Bordetella pertussis is a gram negative bacterium that causes respiratory tract
infection in human (whooping cough). Pertussis toxin (PT) is the main
component of current acellular pertussis vaccine and the S1 (subunit1) is the
main immunogenic part of it. Thus, S1 has been the target of many studies as
a potent candidate of acellular vaccine against Bordetella pertussis, lacking
the side effects of whole cell based ones. S1 gene was amplified and inserted in
three expression vectors including pET-14b, pET-22b(+) and pAED4... |
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Keywords
Bordetella pertussis, Escherichia coli, Pertussis toxin
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